Audiences not working as expected

In the following scenario you can experience unexpected behavior from Audiences Groups:

Assume that your environment includes the following servers:

- SharePoint 2010 (Single Deployment \ Multi Server Deployment):
   - SpsLab1001 (include User Profile Synchronization Service)
- Active Directory Servers:
   - ADLab1
   - ADLab2
   - ADLab3

- NetBIOS Domain Name: LABENV
- FQDN Domain Name: Lab.Env.com


The UPA service configured with Synchronization Connection to Lab.Env.com domain.
On the Manage User Profiles page you can find users with Account Name like this:

lab\userlab1
LABENV\userlab2
lab\userlab3
lab\userlab4

This strange behavior could happen when your NetBIOS Domain name different from your FQDN Domain name like in this scenario.

Because of this, the "Audiences Groups" compile the users who hit the rules with wrong domain name.

As a result of this we can see users who exist inside the audience group and can't see the WebPart that was setup with that audience group.

To fix that we need to enable: NetBIOSDomainNamesEnabled in the UPA with PowerShell.
Please refer to this post: Click Here